Figure 5.

ROS accumulation, activity of antioxidant enzymes, and contents of GSH and AsA in Yugu2 and An04 under salinity. (A) In situ detection of H₂O₂ and O₂⁻ by DAB and NBT staining of 7-day germinated seedlings under salt stress. 7-day-old hydroponic seedlings were treated with 150 mM NaCl for 0 h, 24 h and 48 h, and the roots of each samples were sampled for later use. The activities of the POD (B) and GST (C), and the contents of GSH (D) and AsA (E) were measured. Error bars indicate ± SE of at least three biological repeats. Student’s t-test was carried out to analyze significant differences between two varieties, **indicates the p value < 0.01 and *indicates the p value < 0.05, respectively. The SigmaPlot Version 11.0 was used to draw histograms in B–E.