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. 2020 Aug 12;10:13660. doi: 10.1038/s41598-020-70520-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heat map visualization and PCA of differential metabolites in Yugu2. (A) Heat map visualization. The content of each metabolite was normalized to complete linkage hierarchical clustering. Each example indicated in a single column and each metabolite was showed by a single row. Green and red indicates the down-regulated and up-regulated metabolites, respectively. The color key scale indicated the abundance of metabolites. (B) Score plots for principle components 1 and 2 exhibited great cohesion within groups and good separation between control and salt treatments. (C) The volcano plot indicates the differential metabolites in Yugu2 before and after salt stress. (D) The number of differential metabolites in each class. Pheatmap package of R (v1.0.12, https://cran.r-project.org/web/packages/pheatmap/index.html) was used to create the (A). Ggplot package of R (v3.2.1, https://cran.r-project.org/web/packages/ggplot2/index.html) was used to create the (B). Ropls package of R (v1.18.8, https://bioconductor.org/packages/release/bioc/html/ropls.html)used to create the (C). Microsoft Office Excel (2007) used to create the (D).