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Amplification bias of the multiplex PCR reaction system during primer mix optimization for pool 1. (A) The TRBV amplification bias before optimization. (B,C) The TRBV amplification bias during optimization. (D) The TRBV amplification bias after optimization. (E) The TRBJ amplification bias before optimization. (F,G) The TRBJ amplification bias during optimization. (H) The TRBJ amplification bias after optimization.
