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. 2020 Aug 6;8:452. doi: 10.3389/fped.2020.00452

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Copyright © 2020 Liu, Zhu, Zhu, Wang, Gao and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of Dlk1 and rhNF-κB on the morphology of primary cultured rat type II alveolar epithelial cells (AECII). AECIIs were divided into three groups: (1) the control group, cultured with DMEM, and 12% FBS; (2) the Dlk1 group: cells were cultured with Dlk1 (Abnova, Taipei, Taiwan) at a final concentration of 100 ng/mL; and (3) the rhNF-κB group: cells were cultured with rhNF-κB (Promega, Madison, WI, USA) at a final concentration of 1 gsu/ml. Cells were incubated for 48, 72, and 96 h. Cell morphology was observed under inversion microscope (magnification × 200). Group A = control group; Group B = Dlk1 group; Group C = rhNF-κB group.