Skip to main content
. 2020 Aug 6;11:1611. doi: 10.3389/fimmu.2020.01611

Figure 4.

Figure 4

Effect of corilagin on expression of molecules involved in the TLR4 signaling pathway after ox-LDL stimulation in TLR4-downregulated Ana-1 cells. (A) Expression of TLR4 mRNA was measured by qRT-PCR. *P < 0.05 compared with the control group determined by the Student's t-test (n = 5). (B,C) Abundance of TLR4 protein was measured by Western blotting. *P < 0.05 compared with the control group determined by the Student's t-test (n = 5). (D) mRNA expression of molecules in the TLR4 signaling pathway was measured by qRT-PCR. *P < 0.05 compared with the siRNA-TLR4 group, #P < 0.05 compared with model groups, **P < 0.05 compared with the aspirin group determined by one-way ANOVA and subsequent S–N–K method (n = 5). (E,F) Protein expression was measured by Western blotting. *P < 0.05 compared with the siRNA-TLR4 group, #P < 0.05 compared with the model group (siRNA-TLR4 + ox-LDL group), **P < 0.05 compared with the aspirin group determined by one-way ANOVA and subsequent S–N–K method (n = 5). siRNA-TLR4: TLR4 was knocked down in Ana-1 cells by siRNA. siRNA-NC, siRNA negative control group.