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. 2020 Aug 6;11:1611. doi: 10.3389/fimmu.2020.01611

Figure 5.

Figure 5

Effect of corilagin on expression of molecules involved in the TLR4 signaling pathway after ox-LDL stimulation in TLR4-upregulated Ana-1 cells. (A,B) Upregulation of TLR4 expression in Ana-1 cells via the lentiviral vector. GFP expression was observed with a fluorescence microscope after the lentivirus had been introduced into Ana-1 cells for 72 h. (C) After elimination by puromycin for 7 days, fluorescence was observed. (D) Expression of TLR4 mRNA was measured by qRT-PCR. *P < 0.05 compared with the control group determined by the Student's t-test (n = 5). (E,F) Expression of TLR4 protein was measured by Western blotting. *P < 0.05 compared with the control group determined by the Student's t-test (n = 5). (G) mRNA expression of molecules involved in the TLR4 signaling pathway was measured by qRT-PCR. *P < 0.05 compared with the lentivirus-TLR4 group, #P < 0.05 compared with the model group (lentivirus-TLR4 + ox-LDL group), **P < 0.05 compared with the aspirin group determined by one-way ANOVA and subsequent S–N–K method (n = 5). (H,I) Abundance of the protein of TLR4, TIRAP, MyD88, TRAF6, p38, NEMO, and IRF5 was measured by Western blotting. *P < 0.05 compared with the lentivirus-TLR4 group, #P < 0.05 compared with the model group, **P < 0.05 determined by one-way ANOVA and subsequent S–N–K method (n = 5).