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. 2020 Aug 6;11:1204. doi: 10.3389/fimmu.2020.01204

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Copyright © 2020 Kreimann, Jang, Rong, Greite, von Vietinghoff, Schmitt, Bräsen, Schiffer, Gerstenberg, Vijayan, Dittrich-Breiholz, Wang, Karsten, Gwinner, Haller, Immenschuh and Gueler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neutrophil and macrophage infiltration in kidney tissue. Myeloid cell infiltration was determined after unilateral IRI for 35 and 45 min and compared to sham surgery. 24 h after IRI the majority of infiltrating cells were Gr-1+ granulocytes which were mainly detected in the interstitium of the outer medulla (upper row, GR-1 in red, auto-fluorescence of the tubuli in green, (A,B). At day 7 the most prominent cells were F4/80+ (A,C). No differences between 35 and 45 min IRI were observed (bar: 100 μm, n = 6–8 mice per group, one-way ANOVA, ***p < 0.001).