Figure 4.

DLX6-AS1 downregulates LARGE expression by mediating DNMT1 recruitment to augment the malignant phenotypes of prostate cancer cells. (A) The expression of LARGE in LNCaP and PC-3M cell lines in response to different treatments as measured by RT-qPCR and western blot analysis; (B) the expression of metastasis markers MMP-9, uPAR, and cathepsin B in LNCaP and PC-3M cells under different treatments as assessed by RT-qPCR and western blot analysis; *p < 0.05 vs. cells transduced with oe-NC + sh-NC or oe-NC. Measurement data were summarized as mean ± standard deviation. Unpaired t-test was used for data comparison between the two groups, and comparisons among multiple groups were analyzed by the one-way ANOVA with Tukey's post-hoc test. Cell experiments were repeated three times.