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. 2020 Aug 6;10:1172. doi: 10.3389/fonc.2020.01172

Table 2.

The primer sequences for RT-qPCR in subcellular fractionation.

Target gene Sequences (5′-3′) Target size (bp)
U6 F: 5′-CTCGCTTCGGCAGCACATATAC-3′ 94
R: 5′-AACGCTTCACGAATTTGCGTGTC-3′
12S rRNA F: 5′-TAGAGGAGCCTGTTCTGTAATCGA-3′ 111
R: 5′-TGCGCTTACTTTGTAGCCTTCAT-3′
45S rRNA F: 5′-GAACGGTGGTGTGTCGTT-3′ 130
R: 5′-GCGTCTCGTCTCGTCTCACT-3′

F, forward; R, reverse.