TABLE 3.
List of antibodies for FACS, immunofluorescent staining and western blot analyses.
| Antibody | Relative dilution | Brand | Application |
| CD56-APC | 0.5 μL/1 × 106 cells | BioLegend | FACS |
| Alkaline phospatase-PE | 3.0 μL/1 × 106 cells | R&D Systems | FACS |
| PDGFRa-APC | 2.5 μL/1 × 106 cells | BioLegend | FACS |
| CD15-FITC | 10 μL/1 × 106 cells | BD Pharmingen | FACS |
| CD56 (1) | 1:1000 (mouse) | BD Pharmingen | Immunofluorescence (muscle slide) |
| (2) | 1:300 (mouse) | Immunofluorescence (in vitro staining) | |
| PERILIPIN A/B | 1:200 (rabbit) | Sigma-Aldrich | Immunofluorescence |
| MF20 | 1:20 (mouse) | Hybridoma Bank | Immunofluorescence |
| MYOD | 1:200 (rabbit) | Cell Signaling | Immunofluorescence |
| α Smooth muscle actin | 1:300 (mouse) | Sigma-Aldrich | Immunofluorescence |
| CD90 | 1:100 (rabbit) | Abcam | Immunofluorescence |
| PAX7 | 1:2 (mouse) | Hybridoma Bank | Immunofluorescence |
| LAMININ | 1:250 (rabbit) | Abcam | Immunofluorescence |
| MF20 | 1:4 (mouse) | Hybridoma Bank | Western blot |
| α TUBULIN | 1:1000 (mouse) | Sigma-Aldrich | Western blot |