Figure 1.

Identification of LCPAT1 Upregulation in BC Tissues

(A) Upregulated lncRNAs in BC tissues compared to normal controls using the Cancer RNA-Seq Nexus online tool (http://syslab4.nchu.edu.tw/). (B) Expression value of LCPAT1 according to the Cancer RNA-Seq Nexus online tool. (C) Relative expression of LCPAT1 in 51 BC tissues and their matched normal control tissues by quantitative real-time PCR. (D) Northern blotting analysis of LCPAT1 in four pairs of BC tissues and their matched normal controls. 18S was a loading control. (E) LCPAT1 expression was analyzed in BC tissues with low (n = 31) or high (n = 20) grades. (F) LCPAT1 levels were determined in BC tissues with tumor, node, metastasis (TNM) stage I/II (n = 23) or III/IV (n = 28). (G) Relative expression of LCPAT1 in BC cell lines. (H) Overall survival rate analysis according to LCPAT1 median expression value. (I) Nucleocytoplasmic separation assay was performed to analyze LCPAT1 localization in BC cells. (J) LCPAT1 was mainly located in the nucleus of BC cells as analyzed by a FISH assay. Scale bar, 10 μm. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.