Figure 3.

Overexpression of LCPAT1 Enhances BC Cell Growth and Invasion

(A) Stable overexpression of LCPAT1 was validated by quantitative real-time PCR in MCF-7 and MDA-MB-231 cells. (B) CCK8 assay for proliferation evaluation. (C) EdU assay for analysis of proliferation. (D) LCPAT1 overexpression increased Ki67 expression as shown by IF staining. Scale bar, 100 μm. (E) Colony formation ability was enhanced after LCPAT1 upregulation. (F) Migration analysis by Transwell assay. (G) Invasion detection by Transwell assay. (H) Tumor volumes were measured every 5 days. LCPAT1 overexpression accelerated tumor growth. (I) Tumor weights were determined on day 30. (J) Ki67 expression levels in tumor tissues were analyzed by an IHC assay. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.