Figure 3.

Forced IL-7Rα Expression on Foxp3⁺ Treg Cells

(A) Foxp3⁺ CD4⁺ Tregs from WT and IL-7Rα^Tg mice. Percentage of Foxp3⁺CD25⁺ Treg cells was assessed among the CD4⁺ LN T cells from the indicated mice (left). Bar graph shows a summary of two independent experiments each with four mice (right) and mean with SEM shown. Unpaired two-tailed Student's t test, where NS, not significant.

(B) Surface IL-7Rα expression was assessed on the Foxp3⁺CD25⁺ cells from WT and IL-7Rα^Tg LN CD4⁺ T cells (left). Numbers of IL-7Rα molecules per cell on Foxp3⁺CD25⁺ Treg cells was assessed among the CD4⁺ LN T cells from the indicated mice (right). Bar graph shows summary of three independent experiments each with three mice (right) and mean with SEM shown.

Unpaired two-tailed Student's t test, where ∗∗p < 0.01. See also Figures S4 and S5.