Figure 3.
Nox Is Upregulated in Delaminating Cells
(A) Nox-Gal4 locus and G-TRACE construction.
(B) Snapshots from the movie, z-projections of confocal stacks in the pupal notum of a live fly expressing Nox-Gal4 and G-TRACE (13, 18, and 28 h after puparium formation; APF).
(C) The ratio of cell delamination (from 18 to 28 h APF; three nota, midline (M): 699 DsRed+ cells and 638 DsRed− (GFP+) cells, versus outside the midline (OM): 3,816 DsRed + cells and 3,866 DsRed− (GFP+) cells). Error bars indicate standard error of the mean.
The boundaries between the M and OM regions are outlined with dotted white lines. The anterior-to-posterior axis of the pupa is oriented toward the left. Scale bars: 10 μm.