Figure 1.

Quantitative Comparison of Phenotype-Defined Target CD34 Subsets

(A) Average frequency of CD133⁺, CD38^low/–, and CD90⁺ HSPCs within bulk ssBM-derived CD34⁺ cells (mean ± SD, n ≥ 9 independent healthy donors). (B) Schematic of the quantitative and hierarchical relationship of phenotypic CD34 subsets. (C) Frequency of phenotype-defined subsets in bulk CD34⁺ cells before FACS (top row) and cross-contamination of phenotypic subsets within FACS-purified CD133⁺ (first column), CD38^low/– (second column), and CD90⁺ (third column) HSPCs from a representative ssBM donor used for scRNA-seq analysis. (See also Figure S1.)