Figure 2.

scRNA-Seq of ssBM-Derived CD34 HSPCs and FACS-Purified CD34 Subsets

(A) Dimensional reduction (tSNE) and graph-based clustering (PCA) of scRNA-seq data from ssBM-derived CD34⁺ cells and representative feature plots showing the expression of genes associated with lymphoid-primed, myeloid-primed, erythroid-primed, and immature HSPCs. Level of expression is color coded as shown in the legend in (C). (B) PCA-based transformation with clusters defined in (A) projected onto the PCA analysis and expression of representative genes as shown in (A). (C) Expression of CD34, CD133, CD38, and CD90 in ssBM-derived CD34⁺ cells. Level of expression is color coded as shown in the legend. (D) Overlay of scRNA-seq data from CD34⁺ cells (black, first plot) with FACS-purified CD133⁺ (green, second plot), CD38^low/– (purple, third plot), and CD90⁺ (pink, fourth plot) HSPCs. (See also Figures S2, S3, S4 and S5 as well as Tables S1 and S2.)