Figure 4.

Multilineage Engraftment Potential of Human CD34 Subpopulations

(A and B) Flow cytometric assessment of the frequency of human chimerism in the (A) PB and (B) BM, spleen, and thymus after transplantation of bulk CD34⁺ HSPCs as well as FACS-purified CD34 subpopulations (1 × 10⁵ cells per mouse) from a single G-CSF-mobilized human donor. Engraftment data from a second donor can be found in Figure S9. (C) Frequency of engrafted human CD34⁺ and CD90⁺ HSPCs. CD34⁺ frequency, left y axis; CD90⁺ frequency, right y axis. (D) Human CD34⁺ cells from the murine BM were flow-sorted into CFC assays and erythroid, myeloid, and erythro-myeloid CFC potentials were quantified after 12–14 days. Horizontal line at 0.1% in A and B indicates threshold for the detection of human chimerism. Horizontal bars in (B) and (C) indicate the mean for each population. CFU, colony-forming unit; CFU-M, CFU macrophages; CFU-G, CFU granulocytes; CFU-GM, CFU, granulocytes/macrophages; BFU-E, burst forming unit erythroids; CFU-MIX, CFU erythro-myeloid colonies. (See also Figure S8, S9, and S10.)