Figure 6.

Transplantation of FACS-Purified and Gene-Modified CD90⁺ Cells into NSG Mice

(A) Frequency of human chimerism in the PB over time. (B) Human chimerism in the BM, spleen, and thymus at 20 weeks post-transplant. (C and D) Frequency of GFP⁺ human cells in (C) the PB over time and (D) tissues at 20 weeks post-transplant. (E) Engraftment of human CD34⁺, CD90⁺, and CD90⁺GFP⁺ HSPCs in the BM at necropsy. (F) Human CD34⁺ cells from the murine BM were flow-sorted into CFC assays and erythroid, myeloid, and erythro-myeloid CFC potentials were determined. Horizontal line at 0.1% in A and B indicates the threshold for the detection of human chimerism. Shapes indicate different human donors (n = 3). (See also Figure S11.) Statistics, means ± SEM.