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. 2020 Aug 12;10:13624. doi: 10.1038/s41598-020-64682-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Over-expression of MYCN rescued the decreased malignant phenotype caused by over-expression of miR-98 in NB. (A) The expression of MYCN was detected by western blotting. Representative image from three distinct experiments was illustrated. (B) Cell proliferations of the cells of IMR-32 and SK-N-DZ transfected with miR-NC, miR-98 and miR-98+MYCN at time points of 0 h, 24 h, 48 h and 72 h were analyzed by CCK-8 at the light absorption of the wavelength of 450 nm. (C) Clone formation abilities of the cells of IMR-32 and SK-N-DZ transfected with miR-NC, miR-98 and miR-98+MYCN were analyzed by employing clone formation assay. (D) Migration abilities of the cells of IMR-32 and SK-N-DZ transfected with miR-NC, miR-98 and miR-98+MYCN were analyzed by using Transwell assay without EMC. (E) Invasive abilities of the cells of IMR-32 and SK-N-DZ transfected with miR-NC, miR-98 and miR-98+MYCN were analyzed by using Transwell assay with EMC. Three different experiments have been done. At least three samples were included in an independent experiment.