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. 2020 Aug 12;10:13639. doi: 10.1038/s41598-020-70481-5

Figure 3.

Figure 3

Effect of angiotensin II on PVN-CRF neurons. (a) YFP signal (upper) and regions of interest (ROIs, lower) of a representative brain slice. The brain region is indicated by the red box in the upper illustration. Numbers superimposed over the ROIs align with the dorsoventral axis (from dorsal to ventral). D dorsal, V ventral, L lateral, M medial; scale bar: 100 μm. (b–e) Z-scores of the YFP/CFP ratio recorded from each representative brain slice, (b) and (c) are from the brain slice shown in (a). Upper graphs show traces of individual ROIs (light blue: dorsal PVN, light red: ventral PVN) and mean value (black). Black bars indicate the application timing (2 min) of each substance, angiotensin II (AngII) in (b), (d) and (e), glutamate in (c). Heat maps show the Z-scores of individual ROIs indicated by the color bars at right. Blue and red color bars at left indicate the dorsal (D) and ventral (V) PVN. AngII was applied twice in (d) and (e). The type-1 AngII receptor-selective antagonist losartan was applied 5 min before the second application of AngII in (e). (f) Box plots of ratio of the area under the curve of the Z-scores for the ventral PVN between the first and second application of AngII with and without antagonist. Dots show individual data. ***p < 0.001, Mann–Whitney U test.