Fig. 1.
Increased β-catenin expression in Axin1Osx (KO) mice. a Axin1 expression was significantly decreased in Axin1flox/flox calvarial osteoblasts infected with Adeno-Cre (n = 3, **P < 0.01). b In Axin1flox/flox calvarial osteoblasts infected with Adeno-Cre, Axin1 protein expression was significantly decreased while active β-catenin levels were increased. c Results of IHC showed that Axin1 expression (blue arrowheads) was significantly decreased and β-catenin expression (red arrowheads) was significantly increased on the surface of trabecular bone of newborn Axin1Osx KO mice. d Expression of Axin1 and β-catenin in perivascular cells (red arrowheads) and in adipocytes (green arrowheads) in bone marrow was analyzed by IHC method. e, f Expression of DKK1 (red arrowheads) and Sclerostin (blue arrowheads) in trabecular bone was analyzed by IHC method. g Newborn Axin1Osx KO mice and Cre-negative littermates were collected and whole skeletal Alizarin red/Alcian blue staining was performed. No significant changes in skeletal structure in Axin1Osx KO mice are seen