Leaves of cucumber grown in nutrient solution containing 0.5 mg Ag L−1 as AgNO3 for one week. (A) Light micrograph after XFM analysis, with the white box indicating the area examined by XFM. (B) Tricolor XFM map of Ag (red), Zn (blue), Mn (green) distribution (top panel), with Ag count profile along the white line as shown in the top panel (bottom panel). (C) Fluorescent intensity of XFM spectra collected at 27 keV for areas (A # 1–4), and background (BG #5) as shown in top panel of (B).