Fig. 5.

Characterization of AhR-associated effects in Beas-2B cells. a-b CYP1 activity 48 h post exposure to incinerated thermoplastics and 0.5 μM B[a]P. c-d B[a]P cytotoxicity assessment were conducted using live-cell imaging and membrane permeability (LDH). e Beas-2B cells were treated with graded concentrations of cytochalasin D (CytoD), CH223191, and αNF and assessed for proliferation (WST1; Diamonds) and membrane permeability (LDH; Circles) for co-exposure dose selection. f B[a]P- and incinerated thermoplastic-treated cells were co-incubated with 0.5 μg/mL CytoD, 5 μM CH223191, 10 μM αNF, CH223191 and αNF simultaneously, followed by assessment for WST1 metabolism and ΔΨm. Control bars are not displayed as all data points are normalized to respective controls designated as 100%. Point estimates are the arithmetic mean of 3–4 independent experiments; error bars indicate standard error of the mean (SEM); *p < 0.05, **p < 0.01, ***p < 0.001 compared to respective controls