Table 4.
pSAEC Cytotoxicity Summarization
| Filter Status | Time | Treatment | ED50 (μg/cm2) | ED50P/ED50CNT Potency | ||
|---|---|---|---|---|---|---|
| PI- | WST1 | PI- | WST1 | |||
| Unfiltered | 24 | PC | > 4.0 | > 4.0 | N.D. | N.D. |
| 24 | PC-CNT | > 4.0 | > 4.0 | |||
| 24 | PU | > 4.0 | > 4.0 | N.D. | N.D. | |
| 24 | PU-CNT | 2.21 | > 4.0 | |||
| 48 | PC | > 4.0 | > 4.0 | N.D. | N.D. | |
| 48 | PC-CNT | 2.73 | 3.49 | |||
| 48 | PU | > 4.0 | > 4.0 | N.D. | N.D. | |
| 48 | PU-CNT | 0.83 | > 4.0 | |||
PI- – Cytotoxicity derived from live-cell imaging
WST1 – Cytotoxicity derived from the WST1 method
ED50 – Modeled deposited dose causing 50% of reported cytotoxicity compared to untreated controls
Potency ED50p/ED50CNT – Relative deposited dose causing 50% of cytotoxicity among pristine incinerated thermoplastic (ED50p) compared to CNT-containing analog (ED50CNT) at the same time point. Potency comparisons across time points were not made
N.D. Potency ED50p/ED50CNT value could not be determined as ED50 value for one or both groups exceeded highest concentration tested. None of the filtered preparations caused significant cytotoxicity in pSAECs