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. 2020 Jul 29;5(31):19702–19714. doi: 10.1021/acsomega.0c02396

Figure 3.

Figure 3

Editing NF-κB RELA with TALEN and CRISPR. (A) Schematic HDR donor with two different dual-tagging systems and their tagging cells and target protein. SBP, streptavidin-binding peptide; SAV, streptavidin; HOM, homology arm; UTR, untranslated region; IRES2, internal ribosome entry site 2. (B–D) Editing NF-κB RELA with TALEN and CRISPR in three cell lines and detecting with near-infrared fluorescence (NIRF) imaging. The edited gene was repaired with a homologous donor with two different dual-tagging systems. (B) IRDye800CW-conjugated streptavidin-stained cells in wells. (C) Quantified NIRF intensity of the wells. (D) NIRF imaging of cells collected from wells by trypsinization. Up, NIRF imaging; down, bright field imaging. **p < 0.01 and *p < 0.05.