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. 2020 Jul 29;5(31):19702–19714. doi: 10.1021/acsomega.0c02396

Figure 4.

Figure 4

Editing NF-κB RELA with TALEN and CRISPR. (A) Editing NF-κB RELA with TALEN and CRISPR in HepG2 cells and PANC1 cells and detecting with visible fluorescence. The edited gene was repaired with a homologous donor with two different dual-tagging systems. The cells were stained with Alexa Fluor 488-conjugated Streptavidin and imaged using a fluorescence microscope. The cells were imaged at 100× magnification. (B) Quantitative analysis of the Alexa Fluor 488-conjugated Streptavidin-stained cells by flow cytometry. (C) Statistical results of flow cytometry analysis. ***p < 0.001. (D) Statistical results of ImageJ analysis. ***p < 0.001.