Figure 5.

Editing five NF-κB genes with TALEN and CRISPR. (A) Editing five NF-κB genes with TALEN and CRISPR in HepG2 cells and detecting with visible fluorescence. The edited gene was repaired by the homologous donor of the SBP dual-tagging system. The cells were stained with Alexa Fluor 488-conjugated Streptavidin and imaged using a fluorescence microscope. The cells were imaged at 100× magnification. (B) Quantitative analysis of the Alexa Fluor 488-conjugated Streptavidin-stained cells by flow cytometry. The control cells only transfected with lipofectin showed no fluorescence (Figure S3).