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. 2020 Jul 29;5(31):19702–19714. doi: 10.1021/acsomega.0c02396

Figure 6.

Figure 6

Editing five NF-κB genes with TALEN and CRISPR. (A) Editing five NF-κB genes with TALEN and CRISPR in HepG2 cells and detecting with qPCR. The edited gene was repaired by the homologous donor of the SBP dual-tagging system. The gDNA was prepared from the edited cells and detected by qPCR using a pair of primers, respectively, annealing with the target gene and inserted tag. (B) Visualization of the qPCR products by agarose gel electrophoresis. M1: DL2000 DNA marker (code no. 3427A; Takara). (C) Statistical results of qPCR detection. Fold, the fold of editing efficiency, calculated as (CtTALEN/CtCRISPR) × 10. ***p < 0.001.