Figure 7.

Magnetic isolation of edited cells. The HepG2 cells edited by TALENs targeting five NF-κB genes and repaired by the homologous donor of the SBP dual-tagging system. (A) Cells isolated with streptavidin-coated magnetic beads. (B) and (C) Enriched cells were cultured for 48 h and stained with Alexa Fluor 488-conjugated Streptavidin. The stained cells were imaged using a fluorescence microscope (B) and quantitatively analyzed by flow cytometry (C). (D) Detection of NF-κB RELA tagging by staining nuclei of the edited cells with Alexa Fluor 488-conjugated Streptavidin. The stained cells were imaged using a fluorescence microscope (left) and quantitatively analyzed by flow cytometry (right). The edited cells were treated with TNFα for NF-κB RELA activation. The cells were imaged at 100× magnification.