Figure 1.

Site-specific lipidation of a human IL6-targeting repebody. (A) Structure of the repebody (PDB ID: 4J4L) and a lipidation site on its convex region indicated by a blue color. (B) Chemical structure of 4-azido-l-phenylalanine. (C) Chemical structure of palmitic acid. DBCO-palmitic acid was used for conjugation with 4-azido-l-phenylalanine through copper-free click chemistry. Chemical structures were drawn by using ChemSketch. (D) Schematic diagram of site-specifically lipidated repebody.