Figure 3.
Binding profiles of a palmitic acid-conjugated repebody (Pal-Rb) against human IL-6 and MSA. (A) Binding profile of Pal-Rb against human IL-6. A serially diluted Pal-Rb was added to a human IL-6-coated 96-well plate, and the amount of bound Pal-Rb was measured by direct ELISA. As a control, wild-type human IL-6-specific repebody (WT-Rb) was used. (B) Binding profile of Pal-Rb against MSA. A serially diluted Pal-Rb was added to an MSA-coated 96-well plate, and the amount of bound Pal-Rb was measured by ELISA. WT-Rb and AzF-Rb were used as controls. Error bars describe mean ± standard deviation (n = 3).
