Figure 1.

Synj1 R839C mutation impairs dual phosphatase activities. (A) Domain structure of the brain-enriched isoform of synj1 with their specified functions (on top). Red arrows point to two known Parkinsonism mutations. (B) Normalized lipid conversion, representing enzymatic activities of synj1 R258Q and R839C in comparison with WT synj1. Data obtained using malachite green-based phosphatase assay, from three repetitions. One-way ANOVA was performed for each lipid substrate to compare wildtype (WT) and mutant (RC and RQ) synj1 proteins. P-values are from Turkey’s post-hoc analyses.