Fig. 8.

Protein content of mitochondrial Sirtuins (a) Sirt3, (c) Sirt4, and (e) Sirt5 in plantaris muscle. Representative examples of western blots are shown above each graph. Gel blots are divided because the order of samples in the gel was not equal for the order of groups in results-section. Nonetheless, all gel figures originate from the samples run in the same gel. Western blot gels were run equally as in Fig. 5. To account the effect of intervention on mitochondria, Sirtuins were normalized to mitochondrial markers: cytochrome C [light grey bars in panels: (a) Sirt3, (c) Sirt4, and (e) Sirt5] and citrate synthase activity [(b) Sirt3, (d) Sirt4, and (f) Sirt5]. In the panels, * indicates significant difference to sedentary control (ctrl), * p < 0.05; ** 0.01 > p ≥ 0.001; *** p < 0.001.