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. 2020 Aug 12;40(33):6444–6456. doi: 10.1523/JNEUROSCI.1156-20.2020

Figure 1.

Figure 1.

Expression of IKK2ca led to p65 NF-κB activation specifically in oligodendrocytes. A, PCR analysis using genomic DNA showed that the floxed IKK2ca allele was present in all tissues in IKK2ca cKO mice and WT mice, but the IKK2ca knock-in (KI) allele was only present in the CNS of IKK2ca cKI mice. 1, brain; 2, SP; 3, heart; 4, liver; 5, spleen; 6, lung. B, Western blot analysis showed that Flag-IKK2ca was detectable in the brain of eight-week-old IKK2ca cKI mice but not in WT mice. C–I, CC1 and Flag double immunostaining showed that the immunoreactivity of Flag-IKK2ca became detectable selectively in oligodendrocytes in the brain of eight-week-old IKK2ca cKI mice, as compared with WT mice. J–P, ASPA and the active form of p65 double immunostaining showed that the number of oligodendrocytes that stained positive for the active form of p65 was dramatically increased in the brain of eight-week-old IKK2ca cKI mice compared with WT mice. N = 3 animals. Scale bars: 20 μm. Statistical analyses were done with a t test. Error bars represented SD; *p < 0.05.