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. 2020 Aug 12;40(33):6444–6456. doi: 10.1523/JNEUROSCI.1156-20.2020

Figure 3.

Figure 3.

Enhanced NF-κB activation specifically in oligodendrocytes rendered mice resistant to EAE and abrogated the increased susceptibility of PERK cKO mice to EAE. A, Mean EAE clinical score. Error bars represent SEM. B, The mean aggregate EAE clinical score was significantly decreased in IKK2ca cKI mice (N = 16 animals) but significantly increased in PERK cKO mice (N = 16 animals), as compared with WT mice (N = 20 animals). The mean aggregate EAE clinical score of Double mice (N = 16 animals) was significantly decreased as compared with PERK cKO mice and WT mice. Error bars represent SD. C–G, ASPA and the active form of p65 double immunostaining showed that the number of oligodendrocytes (arrows) that stained positive for the active form of p65 was significantly increased in the lumbar SP of IKK2ca cKI mice and Double mice compared with WT mice at PID19 and was significantly decreased in the lumbar SP of PERK cKO mice compared with WT mice and Double mice at PID19. N = 4 animals. Error bars represent SD. Scale bars: 20 μm (C–F). Statistical analyses were done with a one-way ANOVA with a Tukey's post hoc test; *p < 0.05.