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. 2020 Aug 12;40(33):6270–6288. doi: 10.1523/JNEUROSCI.0925-20.2020

Figure 1.

Figure 1.

Identification of tonic Ib and phasic Is motoneuron GAL4 drivers. A, Comparison of synaptic and biophysical properties of Ib and Is motoneurons in Drosophila larvae. B, Confocal image of UAS-CD8-GFP driven by MN1-Ib GAL4 (GMR94G06) in the third instar larval VNC from the FlyLight Project GAL4 collection. Arrows denote the paired MN1-Ib cell bodies in each abdominal segment, and arrowheads denote GFP expression in axons exiting the VNC. Scale bar, 50 µm. C, Diagram of MN1-Ib innervation in a larval abdominal hemisegment. D, Immunostaining for anti-GFP (green) to label MN1-Ib and HRP (magenta) to label all axons in a MN1-Ib GAL4; UAS-CD8-GFP third instar larva. Muscles M1 and M2 are indicated. Scale bar, 20 µm. E, Diagram of MNISN-Is and MNSNb/d-Is innervation in a larval abdominal hemisegment. F, Immunostaining for anti-GFP (green) to label MNIs and HRP (magenta) to label all axons in a MNIs GAL4 (6-58); UAS-CD8-GFP third instar larva. Muscles M1 and M2 are indicated. Scale bar, 20 µm.