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. 2020 Aug 12;40(33):6409–6427. doi: 10.1523/JNEUROSCI.0724-20.2020

Figure 2.

Figure 2.

Activity of VTA TH neurons during relapse. A, Cre-dependent gCaMP6f was applied to the VTA. Location of gCaMP6 expression and fiber tips in midbrain with each rat represented at 25% opacity. The lateral edge of the fasciculus retroflexus was used as anatomic boundary between mVTA and lVTA. Example of gCaMP6 expression in VTA TH neurons and a representative trace is shown. B, Mean ± SEM active (A) and inactive (I) nosepokes from the five recording sessions. C, Mean ± SEM calcium transients in VTA TH neurons ±3 s around Nosepoke+ (active nosepokes that triggered the pump) and Nosepoke– (active nosepokes at other times) for mVTA and lVTA. Colored bars above traces represent periods (with minimum consecutive threshold) of significant difference from 0 as defined by 95% CIs. D, Mean ± SEM calcium transients in VTA TH neurons from –3 s to 3 s around Magazine+ (first magazine entry after Nosepoke+) and Magazine– (magazine entries at other times) for mVTA and lVTA. Colored bars above traces represent periods (with minimum consecutive threshold) of significant difference from 0 as defined by 95% CIs.