Table 2.
qPCR assay and sequence information for DNA of each of 9 targeted species.
| Species | Forward primer name/Sequence | Reverse primer name/Sequence | Probe name/Sequence with labeling | Gene location | GenBank accession number | Amplicon size (bp) |
|---|---|---|---|---|---|---|
| Beef | B.taurusHBB-1084f GTTGTGCTGGCTCAGCCTGCA | B.taurusHBB-1147r CCTTCTGAAAGTCGCAAT | B.taurusHBB-1103p FAM- TGGCAAGGAATTCA-MGB | HBB | X00376 | 64 |
| Chicken | GallusBactin-3323f CAGCTGGCCTGCCGG | GallusBactin-3419r AAAGGACCAGGACCAGTATTGC | TGGCAGAG | TGF-BETA3 | X60091 | 97 |
| Duck | CairinaBactin-1588f CTTTGATTTGTTCAGCGAGCG | CairinaBactin-1710r TCAGTGTACAGGTAGCCCCTCTCT | CairinaBactin-1656p FAM-CCTGCCTAGGAGAGGT-MGB | beta actin | NM_001310421 | 123 |
| Turkey | Meleagris-303f CGGCCTATATTATGGTTCGTACCT | Meleagris-398r ACATAGCCTACAAAGGCTGTTGC | Meleagris-354p FAM-AGTCTTACTTCTCACCCTC-MGB | cytochrome b | L08381 | 96 |
| Salmon | Oncorhynchus-1453f CCCTGTTGCCTGATGGAGTC | Oncorhynchus-1534r ATTGGGCTCACGATGGAGTC | Oncorhynchus-1479p FAM-CTGAACAAGATATTCCTG-MGB | growth hormone 1 | X61938 | 82 |
| Sheep | O.ariescytoB-513f CAAAGCTACCCTCACCCGATT | O.ariescytoB-642r GTCCGATGGAATTCCTGTGG | O.ariescytoB-573p FAM-CCTCGCCATAGTTCACCT-MGB | cytochrome b | KY110724 | 130 |
| Rabbit | LeporidaeBactin-74f CCATGGATGACGATATCGCC | LeporidaeBactin-150r ATCGTCGCCCGCGAA | LeporidaeBactin-99p FAM-CGTGGTCGACAATGG-MGB | beta actin | NM_001101683 | 77 |
| Kangaroo | MacropusNADH-118f GTAGGCCCCTACGGACTCCT | MacropusNADH-237r GAGGGCTAGGATTGGGGC | MacropusNADH-177p FAM-ACCCCTACGACCTTT-MGB | NADH | JN003396.1 | 120 |
| Pig | SusBactin-297f GACGAGGCTGCCGTAAAGG | SusBactin-406r TGCAAGGAACACGGCTAAGTG | SusBactin-352p FAM- TCTGACGTGACTCCCCGA-MGB | Beta actin | DQ452569 | 110 |