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. Author manuscript; available in PMC: 2020 Aug 13.
Published in final edited form as: Methods Enzymol. 2019 Jun 29;635:81–93. doi: 10.1016/bs.mie.2019.05.038

Figure 1:

Figure 1:

Tissue specimens were obtained stained for immunofluorescent microscopy as outlined in Sections 3.1-3.3 and Tables 1-3. Images were scanned at 10x and regions of interest (shown in blue) were selected on spectrally uncompensated images. (a-c) Spectrally uncompensated images from a single GL261-luc2 tumor (a), B16-OVA melanoma (b), and Spleen (c). Scale bar = 800 μm. B16-OVA tumor specimens were courtesy of Anthony Rodriguez and Dr. Victor Engelhard; protocols for tumor generation and isolation are published (Rodriguez et al., 2018).