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Tertiary Lymphoid Structures Identification Staining Panel
| Staining Order |
Antigen Retrieval |
Primary Antibody and working dilution |
Secondary Antibody |
OPAL |
|---|---|---|---|---|
| 1. | AR9 | PNAd (1:50) | Super Picture HRP | 620 |
| 2. | AR9 | CD4 (1:100) | Opal Polymer HRP | 520 |
| 3. | AR9 | CD8 (1:200) | Opal Polymer HRP | 540 |
| 4. | AR9 | CD19 (1:50) | Opal Polymer HRP | 650 |
| 5. | AR9 | CD11c (1:100) | Opal Polymer HRP | 570 |
| 6. | AR9 | CD34 (1:100) | Opal Polymer HRP | 690 |
| 7. | AR9 | - | Opal Polymer HRP | Spectral DAPI |
