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. 2020 Aug 12;11:352. doi: 10.1186/s13287-020-01869-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — MSC repair wound healing capacity, barrier function, and angiogenic properties of injured HUVEC. a HUVEC monolayer permeability was increased after injury. MSC at a 1:2 ratio reduced HUVEC monolayer permeability by 44% after 24 h of incubation. Incubation with HI-MSC or MSC through a transwell had no effect. b Hypoxia-reoxygenation injury decreased the wound healing capacity of HUVEC measured by a scratch assay. After 24 h incubation with MSC at a 1:2 ratio, MSC improved HUVEC capacity to close a scratched area by 45%. Secreted molecules by MSC during incubation through a transwell improved injured HUVEC wound healing by 22%. At a ratio of 1:10 MSC-HUVEC, wound healing capacity was improved by 17% by MSC. HI-MSC had no effect at any ratio. c The total length of tube-like structures formed by HUVEC was measured to quantify angiogenic capacity. Hypoxia and reoxygenation injury decreased HUVEC angiogenic potential by half. After 24 h of incubation with MSC at a 1:2 ratio, injured HUVEC fully recovered their angiogenic capacity. At a 1:10 ratio, MSC improved angiogenic potential of injured HUVEC by 50%. Secreted molecules by MSC at a 1:2 ratio led to a 48% recovery on injured HUVEC angiogenic potential. HI-MSC had no effect at any ratio. d Schematic depiction of endothelial monolayer integrity. Added FITC-conjugated dextran leaks through an injured endothelial monolayer. e Visual representation of non-injured (left) and injured (right) HUVEC angiogenic potential. f Visual representation of HUVEC wound healing capacity. Top panel: a scratch is made to the endothelial monolayer at time point 0 h. Bottom left panel: non-injured HUVEC completely close the scratch after 6 h. Bottom right panel: injured HUVEC are not able to completely close the scratch after 6 h (n = 5). Results are shown as mean ± SD. Significance of the comparison between injured HUVEC and the effect of MSC is shown as **p value < 0.01 and *p value < 0.05