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. 2020 Aug 12;16:289. doi: 10.1186/s12917-020-02516-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Gene expression of endocannabinoid enzymes in duodenal and ileal mucosa of piglets. Data are expressed as means (n = 8) and S.E.M. represented by vertical bars. a, b Values with different superscripts differ significantly at P < 0.05. T1 = basal diet; T2 = basal diet + 25.5 mg of thymol/kg feed; T3 = basal diet + 51 mg of thymol/kg feed; T4 = basal diet + 153 mg of thymol/kg feed; T5 = basal diet + 510 mg of thymol/kg feed (Vetagro SpA, Reggio Emilia, Italy). A modification of the 2–ΔΔCT method was used to analyze the relative expression (fold changes), calculated relative to the control group (control; Livak and Schmittgen, 2001 [21]). DGL-α = diacylglycerol lipase alpha; DGL-β = diacylglycerol lipase beta; FAAH = fatty acid amide hydrolase