Table 2.
Primer sequence used for gene expression analysis
| Gene | Primer sequence (F and R) 5′ → 3′ | Product length (bp) | Accession N. | Reference |
|---|---|---|---|---|
| CB1 | F: TTCCCCACTTCTTTTCCGCC R: GGGAGTCCCTTCGCATCC | 208 | XM_013992672.2 | Present study |
| CB2 | F: TTTATAGCCTGGCCTCCCCT R: TTTTCCCGTCTGCCTCTGTC | 240 | XM_021095530.1 | [50] |
| FAAH | F: TGCCACCGTGCAAGAAAATG R: CCACTGCCCTAACAACGACT | 234 | XM_013999418.2 | [50] |
| DGL-α | F: GAAACCAAACACGCCTCCAC R: CAACCCAGCAGCAAAGGAAC | 211 | XM_021082924.1 | [50] |
| DGL-β | F: TTTGTAATCCCGGACCACGG R: GACCTGCCGAGGAATACGGA | 255 | XM_021086077.1 | Present study |
| TRPV1 | F: TCACCAACAAGAAGGGGCTC R: GGATAGGTGCCTGCACTCAG | 116 | XM_005669121.1 | [51] |
| OR1G1 | F: CTTGGTTTGTGTGCTCTGCC R: GAAAAGGCTTTCCGCTTCCC | 96 | XM_013990010.1 | Present study |
| INF-γ | F: GGCCATTCAAAGGAGCATGGATGT R: TGAGTTCACTGATGGCTTTGCGCT | 149 | NM_213948.1 | [52] |
| TNF-α | F: GCCCACGTTGTAGCCAATGTCAAA R: GTTGTCTTTCAGCTTCACGCCGTT | 99 | NM_214022.1 | [52] |
| RPL35 | F: AACCAGACCCAGAAAGAGAAC R: TTCCGCTGCTGCTTCTTG | 146 | NM_214326.2 | [53] |
F forward, R reverse, CB1 cannabinoid receptor 1, CB2 cannabinoid receptor 2, FAAH fatty acid amide hydrolase, DGL-α diacylglycerol lipase alpha, DGL-β diacylglycerol lipase beta, TRPV1 transient receptor potential vanilloid 1, OR1G1 olfactory receptor 1G1, IFN-γ interferon-γ, TNF-α tumor necrosis factor-α, RPL35 ribosomal protein L35.