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. 2020 Aug 4;12:6837–6847. doi: 10.2147/CMAR.S256624

Figure 3.

Figure 3

MicroRNA (MiR)-29c-3p was a target gene of taurine upregulated gene 1 (TUG1) in hepatocellular carcinoma (HCC). (A) Bioinformatics analysis showed the predicted binding site between TUG1 and miR-29c-3p. (B) Relative expression of miR-29c-3p in HeP3B and sk-Hep-1 cells transfected with si-NC or si-TUG1-1 was measured by quantitative reverse-transcription PCR (qRT-PCR). **P < 0.01 vs the si-NC group. (C, D) RNA-binding protein immunoprecipitation (RIP) and dual-luciferase reporter (DLR) assays were used to verify the targeting relationship between the TUG1 and miR-29c-3p. C, **P < 0.01 vs the anti-IgG group; D, **P < 0.01 vs the miR-NC group. (E) The expression of miR-29c-3p in 51 paired HCC tissues and matched adjacent non-tumor tissues was examined by qRT-PCR. **P < 0.001. (F) Correlation analysis between the expression of miR-29c-3p and TUG1. P = 0.0004, r = −0.4735. (G) The expression of miR-29c-3p in four HCC cell lines (sk-Hep-1, HeP3B, HepG2 and SMMC-7721) and normal human liver cell line L-02 was assessed by qRT-PCR. **P < 0.01 vs the L-02 cells group. Data were represented as mean ± standard deviation (SD) of at least two independent experiments.