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. 2020 Aug 4;12:6837–6847. doi: 10.2147/CMAR.S256624

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© 2020 Zhao et al.

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MicroRNA (MiR)-29c-3p inhibited the proliferation, migration and invasion of hepatocellular carcinoma (HCC) cells. HeP3B and sk-Hep-1 cells were transfected with miR-negative control (NC), miR-29c-3p mimics or miR-29c-3p inhibitors. (A) Quantitative reverse-transcription PCR (qRT-PCR) was used to detect the expression of miR-29c-3p. (B) 3-(4, 5-Dimethyl-2-Thiazolyl)-2, 5-Diphenyl-2-H-Tetrazolium Bromide (MTT) assay was applied to measure cell proliferation at 0, 24, 48, 72 and 96 h in transfected HeP3B and sk-Hep-1 cells. (C) Wound-healing assay was conducted to evaluate cell migration of transfected HeP3B and sk-Hep-1 cells. (D) Transwell invasion assay was used to detect the invasion of HeP3B and sk-Hep-1 cells. Scale bar = 100 μm, ×400 magnification. **P < 0.01 vs the miR-NC group. Data were represented as mean ± standard deviation (SD) of at least two independent experiments.