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. 2020 Aug 5;14:3143–3158. doi: 10.2147/DDDT.S255828

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© 2020 Duan et al.

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IRAK1 and NFAT5 were target genes of miR-146a-5p. (A) Expression level of miR-146a-5p in the brain tissue of rats. (B and C) Expression level of IRAK1 and NFAT5 protein in the brain tissue of rats. (D) A photograph depicted targeting sites of miR-146a-5p on IRAK1 and NFAT5 mRNA. (E and F) Dual-luciferase assay was performed to test binding between miR-146a-5p/IRAK1 and miR-146a-5p/NFAT5. The data for the expression of miR-146a-5p, IRAK1 and NFAT5 protein were derived from six rats in each group. The data are presented as the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001.