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. 2020 Aug 12;20:251. doi: 10.1186/s12866-020-01934-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — The detection limit, sensitivity, and precision of Coxiella-UPT-LF. a Photograph of a UPT-LF strip. b Photograph of the UPT biosensor. c Illustrations of the UPT assay for the detection of C. burnetii Xinqiao strain. Peaks on the left are signals for the control bands, and peaks on the right are for test bands. d LPS immunoblot of C. burnetii NMI and NMII. LPS of C. burnetii NMI and NMII was separated by SDS-PAGE, silver-stained, and probed with PI LPS-specific mAbs (10B5 and 10G7). e Standard curve for the quantification of C. burnetii Xinqiao strain by Coxiella-UPT-LF, with the logarithm of the difference between the T/C ratio and the cut-off value on the horizontal axis and the logarithm of the concentration (GE/ml) on the vertical axis. Data were expressed as mean ± SD, n = 3. f Standard curve for the quantification of NMI LPS by Coxiella-UPT-LF, with the logarithm of the difference between the T/C ratio and the cut-off value on the horizontal axis and the logarithm of the concentration (GE/ml) on the vertical axis. Data were expressed as mean ± SD, n = 3