Figure 1. Caspase-6 Promotes IAV-Induced ZBP1-Mediated NLRP3 Inflammasome Activation.

(A) Immunoblots analysis of pro- (p45, black arrow) and cleaved caspase-1 (p20, red arrow; CASP1) in LPS plus ATP-treated bone-marrow-derived macrophages (BMDMs).

(B) Assessment of IL-18 release by ELISA following LPS plus ATP treatment.

(C) Assessment of IL-1β release by ELISA following LPS plus ATP treatment.

(D) Images of BMDMs after LPS plus ATP treatment.

(E–H) Immunoblot analysis of CASP1 (E), IL-18 release (F), IL-1β release (G), and cell images (H) from BMDMs primed with LPS and then transfected with LPS.

(I–L) Immunoblot analysis of CASP1 (I), IL-18 release (J), IL-1β release (K), and cell images (L) from BMDMs after influenza A virus (IAV) infection for 16 h.

(M–P) Immunoblot analysis of CASP1 (M), IL-18 release (N), IL-β release (O), and cell images (P) from BMDMs after P. aeruginosa (MOI, 2) infection for 2–3 h.

(Q–T) Immunoblot analysis of CASP1 (Q), IL-18 release (R), IL-1β release (S), and cell images (T) of BMDMs after F. novicida (MOI, 100) infection for 20 h.

The red arrows indicate pyroptotic cells (D, H, L, P, T). NS, not significant; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA). Data are representative of three independent experiments. Data are shown as mean ± SEM (B, C, F, G, J, K, N, O, R, and S). The original magnification of all images is 320. See also Figure S1.