Figure S1. RT-qPCR analysis of the spo0E-like gene expression in P. polymyxa.

Normalized RNA levels of the spo0E-like gene in P. polymyxa (PPTDRAFT_04974) were measured with RT-qPCR, after treatment with 5 μM AloP13 mature peptide (SHGRGG, gray bars), AloP13 scrambled mature peptide (GRGSGH, blue bars), or no added peptide (green bars). Each experiment was done in two biological replicates and measured with two sets of primers matching different regions of the gene sequence (see the Materials and Methods section). (A) Primers set #1. (B) Primers set #2. For (A, B), error bars indicate SD of 2–3 measurements for each sample, and the housekeeping gene gyrB was used for normalization. Relative mRNA levels were calculated compared to “no peptide repeat A.”