Primers used in this study.

Name	Sequence	Use
Spo0E_1_F	TGGCGAAAGAGGTGGGATTGA	RT-qPCR of spo0E-like gene (PPTDRAFT_04974)—primers set 1
Spo0E_1_R	GGGTACGCTCTGTTGTCTACGA	RT-qPCR of spo0E-like gene (PPTDRAFT_04974)—primers set 1
Spo0E_2_F	GAGTTGGATCGGCTCCTTAAT	RT-qPCR of spo0E-like gene (PPTDRAFT_04974)—primers set 2
Spo0E_2_R	CTCGTGTTGCTTCTCTTGGA	RT-qPCR of spo0E-like gene (PPTDRAFT_04974)—primers set 2
gyrB_F	GCCAGCGATACATTCCACTAT	RT-qPCR of gyrB gene for normalization
gyrB_R	CACGAGAGCCTTCGACATAAA	RT-qPCR of gyrB gene for normalization
Alo13_F	ATGGAAAATGCAACCACGATTC	Used to amplify the Alo13 locus for cloning (start of AloR13 ORF)
Alo13_R	ATCAATAAACCTCCTGTTCGGTG	Used to amplify the Alo13 locus for cloning (end of Spo0E promoter)
Alo13_Gib_F	CACCGAACAGGAGGTTTATTGATatgtcaaaaggagaagaactttttacag	Used for Gibson cloning of Alo13_F and Alo13_R amplified fragment (lower case letters correspond to beginning of sfGFP)
Alo13_Gib_R	GAACGAATCGTGGTTGCATTTTCCATgtttgtcctccttattagttaatcagc	Used for Gibson cloning of Alo13_F and Alo13_R amplified fragment (lower case letters correspond to RBS sequence following hyper-spank promoter)
AloP13_mut_F	TGTGCAAAGACGAAGAAGGTTTTTC	Used for site-directed mutagenesis of AloP13 start codon ATG > ACG
AloP13_mut_R	CCCCCTTAATAATAGATTTATAAATTTC	Used for site-directed mutagenesis of AloP13 start codon ATG > ACG